EDTA tubes are coated with K2-EDTA or K3-EDTA to block the coagulation cascade by binding calcium ions in the blood sample. EDTA tubes should be inverted 8~10 times to ensure complete mixing with the blood for a correct coagulation value. EDTA tubes are used for direct sampling analysis because erythrocytes and leukocytes are stable for 24 hours with EDTA while thrombocytes are stable for 6~8 hours.
The blood smear should be performed within 3 hours of blood collection. EDTA tubes are intended for whole blood examination in hematology.