Fertility test kit BRED-013

sperm

In normal circumstances, the basic protein (nucleoprotein) bound to the sperm nucleus DNA will undergo the process of natural maturity from the histone to protamine, of which, such substitute process of histone by protamine is called nucleoprotein transition, which has an important physiological significance. The spermatozoid nucleus carries all genetic information from paternal, and these genes must start the expression after the fertilization. Before the fertilization, the sperm genes are specially protected by the protamine and closely concentrate with no DNA transcription. However, the abnormality of nucleoprotein transition may cause the male infertility or early embryo abortion. The mechanism lies in: a) the instability in sperm DNA and easily to be damaged and difficult to get pregnant; b) once fertilized, the spermatozoid nucleus cannot normally depolymerized due to the nucleoprotein pattern, and thereby to influence the binding of female and male pronuclei; c) the abnormal development of embryo may induce to the embryo abortion. Instructions: The reagent is composed of aniline blue, acetic acid, phosphate buffer and preservative. Each composition in kits in different lots can be used interchangeably. 【Storage conditions and duration】 1. Storage conditions The kit should be kept out of sun and stored at the temperature of 2~8° C; the tissue slide, cover slip and slide barrel can be stored at the room temperature. 2. Duration: The kit should be stored at the temperature of 2~8° C, with a use life of 12 months. Once unsealed, the kit would be kept for 45 days at 2~8 ° C.