Cryopreservation of mammalian cells is extremely valuable and very common in biological research. Fear of losing a cell line to contamination or incubator failure is frequently the impetus for making archival storage of cells a high priority after receiving or generating a new cell line. While there are numerous protocols for freezing cells, all involve the use of a ‘freezing medium’ containing a cryoprotectant. Once transferred from growth media to freezing medium, the cells are usually frozen at a controlled rate and stored in liquid nitrogen vapor or at -130°C in a mechanical deep-freeze. Although freezing a cell line is a commonly-performed procedure, problems arise when suitable freezer space is not available, or when unwanted variables are introduced by the presence of serum, extra washes, or complicated freezing algorithms.
These problems have been resolved by the family of Bambanker Cell Freezing Media reagents. The unique technology of Bambanker permits cryopreservation of cells at -80°C or in liquid nitrogen, obviating the need for an additional and expensive ultra-low freezer and avoiding time consuming and complicated controlled freezing protocols.
Higher number of intact, healthy, and undifferentiated cells after thawing
How much are your cells worth? The family of Bambanker freezing reagents offer the unique combination of simplicity and high survivability. The results can be miraculous. These products have been used extensively across the globe. Developed in Japan, Bambanker reagents have been validated on well over 1,000 different cell lines at the JCRB. You can also see these recent published references as a testament to its successful use in labs.