Dye reagent kit Nano-Booster

FISHproteinlead

Fluorescent proteins are a powerful tool to study protein localization and dynamics in living cells. However, being genetically encoded and not chemically engineered, proteins such as Green Fluorescent Protein (from jellyfish) and Red Fluorescent Protein (from mushroom coral) inherit some general shortcomings. Signal intensities of fixed samples from cells expressing a GFP- or RFP-fusions at physiological expression levels are usually very low. Also, the photostability and the quantum efficiency of these chimeric proteins are not sufficient for Super Resolution Microscopy (e.g. 3D-SIM or STED). Many cell biological methods such as HCl treatment for BrdU-detection, the EdU-Click-iT™ treatment or heat denaturation for FiSH lead to disruption of GFP or RFP signal. GFP-Boosters and RFP-Boosters are very small, binding proteins highly specific for GFP or RFP and covalently coupled to the superior fluorescent dyes from ATTO-TEC. The GFP/RFP binding domain is derived from the proprietary Alpaca-produced Nanobodies used in our popular Nanobody-Trap Affinity Reagents. Nano-Boosters provide a much stronger and more stable signal of ONLY your fusion proteins in fixed cells. Both GFP-Booster and RFP Booster are linked to different and very efficient dyes ranging from green, to red, right up to far red in emission. Choose the one that is perfect for your study. These varieties allows for many more post-growth analyses than previously thought possible for GFP, RFP, Histone, and Vimentin fusion proteins.