The most popular transfection procedures all require specialized chemicals, which are costly—as much as $8, $12, or even $16 or more per sample! These additives can have unwanted effects on cells; at the very best, they create another parameter to be dealt with. Though poking holes in cell membranes has been done for many years, no protocols exist for high efficiency transfections without the use of such reagents. That is, not until the NEPA21. Nepa Gene has created THE most sophisticated apparatus for delivering DNA and RNA to cells—and all that’s needed are the electrodes. With a combination of low voltage short-and-long pulses and reversing polarities, a huge variety of cells, tissues, and even whole organisms can be transfected with amazingly high efficiencies. This patented pulsing technology has been shown to be both superior to and far more flexible than other techniques. Relying upon unique non-capacitor driven pulsing, the NEPA21 works at low voltages to deliver perfect square waves with no degradation. Results with the NEPA21 are often far better than those with cheaper models, especially when it matters most. And because everything works at low voltages, cytotoxic effects are nearly eliminated! Read more about the secrets behind NEPA21’s proprietary pulsing technology below.
In vitro transfection of suspension, adherent, and primary cells
The NEPA 21 utilizes one of two electrode types to deliver nucleic acids to live cells in culture. The first type, inexpensive electroporation cuvettes, can be used on suspension cells without the need for expensive buffering conditions with unknown additives. Just add cells and pulse–then save time and money.