Cell electroporator NEPA Porator

Electroporation is a process by which one or more electrical pulses are delivered to a biological sample. These pulses result in the temporary disruption of the cell membrane, and under some conditions the cell wall. The “porated” cells are then able to quickly take up DNA, RNA, or proteins in the surrounding buffer. The NEPA Porator is designed to deliver one or two DC exponential decay waves with constant voltage. What makes the NEPA Porator so special is its ability to first check the resistance of the sample via a trickle charge. This quick check ensures your samples’ buffering conditions are in the right range, so you know that you’re applying the correct amount of energy each and every time. In many cases, a quick adjustment to the sample volume in the cuvette can bring resistance back into the optimal range so that you can proceed with your experiment. In other cases, the system helps to identify “dirty” cells or DNA preps, so that these components can be re-purified and electroporation can move forward with the promise of consistent results. Simple functionality without arcing In bacterial electroporation, trace amounts of salts often drive down resistance and result in the scary popping noise referred to as “sparking” or “arcing”. This can also happen when bacterial cells are over-porated, causing them to release their intracellular contents; the cascading drop in resistance that follows can ruin whole experiments. The NEPA Porator helps eliminate arcing by allowing you to “see” a drop in resistance before electroporation even begins!