DNA purification reagent kit 17095

for PCR

The Fast DNA-spin Plasmid Purification Kit employs a simple two-step process – break open walled cells and purify plasmid DNA. Based on the proven technology of optimized alkali lysis buffering system and silica membrane, this kit will isolate extremely pure plasmid DNA from bacteria. Its big advancement over the standard DNA-spin Plasmid Purfication Kit is that it uses a new lysis buffer allowing the adsorption of DNA onto silica membrane in the presence of high salt. Now purify DNA in under 12 minutes (vs 20-30 minutes) with yields that are 20-30% higher. The Fast DNA-spin kit also includes a neutralization buffering technique for gentle pH conditions during binding. So stabilize the isolation and purification steps, while also efficiently removing chromosomal DNA and cell debris…particularly plasmids larger than 30 Kb. The novel “Lysis Viewer Reagent” is added to the reaction for a visual pH confirmation. Different colors indicate proper lysis and neutralization, making step protocol steps plain to see. The Fast DNA-spin Plasmid DNA Purification Kit can be used for both high-copy and low-copy plasmids so it is perfect for all your small-scale plasmid purification needs. KIT CONTENTS 200 Column Kit Resuspension Buffer - 60 mL Lysis Buffer - 60 mL Neutralization Buffer - 80 mL Washing Buffer - 50 mL Elution Buffer - 30 mL Spin Columns - 200 tubes Collection Tubes - 200 tubes RNAase A, 10mg/ml - 600 µL Lysis Viewer - 300 µL Storage - Room Temperature Shelf Life - 1 Year