The Cairn Alligator system allows bioluminescence measurements to be simultaneously recorded from up to six multi-well plates over several days. Based on the New Brunswick Galaxy 170R, we make substantial modifications to convert the standard incubator into a high throughput luminometer. In addition to the precise temperature, CO2 control is provided as standard – we adapt the chamber for light-tightness and high NA imaging using a deep-cooled, back-thinned or Electron Multiplied CCD. The system is supplied fully integrated into various software packages for multi-region timelapse analysis.
Additional information
Applications
• Circadian rhythm measurements – seconds to weeks
• Calcium recordings (Aequorin)
• Plant sciences
Key Benefits
• High throughput, up to six 384 well plates imaged simultaneously
• Precise environmental control
• LED illumination for circadian rhythm stimulation
• High aperture lens with demisting optics
• Light-tight, does not require a dark room
• Software control of all environmental conditions and illumination
Specification
The Alligator system includes the following modifications:
• Secondary shroud for complete light tightness
• F/0.95 lens with anti-mist heater
• Anti-reflective stainless steel racks
• Multi well plate alignment
• External focus adjustment
• Photon trap of CO2 sensor
• Illumination ring
• Supported in various software packages
• Custom scripting for multi-well recordings and data export
• Integrated deep-cooled camera from Hamamatsu, Andor or Teledyne