Prolactin reagent kit PR063F-100

polyclonal antibodyfor researchprotein

Prolactin (lactogenic hormone) is a single chain polypeptide hormone with a molecular weight of approximately 23,000 daltons. Prolactin is secreted from the anterior pituitary gland. Plasma and pituitary PRL levels are significantly greater in adult female than in male rats. This difference is thought to be brought about by ovarian steroids. A similar sex difference was found in pituitary PRL content in normal mice from 30 days of age. During and following pregnancy, prolactin, in association with other hormones, stimulates breast development and milk production. Hypersecretion of prolactin can be caused by pituitary tumors, hypothalamic diseases, hypothyroidm, renal failure, acute exercise and several medications. Quantity 96 Tests (12x8 breakable strip wells) Standard range - 10-800 ng/ml Analytical Sensitivity 10 ng/ml Sample volume - 10 µl/well Species - Mouse or Rat Application - The Calbiotech Mouse/Rat Prolactin ELISA kit is used for the quantitative measurement of prolactin in Mouse or Rat serum or plasma. Principle - The Calbiotech Mouse/Rat Prolactin kit is based on a solid phase sandwich ELISA. The samples and diluted biotinylated anti-prolactin antibodies are added to the wells coated with polyclonal antibody to prolactin. Prolactin in samples binds to the anti-prolactin polyclonal antibody on the well and the biotinylated antiprolactin antibody binds to prolactin. Unbound protein and biotin conjugate are washed off by wash buffer. Precautions Not for use in diagnostic procedure