IgA reagent kit SC221A

solutionfor researchELISA test

Quantity 96 Tests (12x8 breakable strip wells) Standard range - 6.25-200 µlg/dl Analytical Sensitivity 6.25 µlg/dl Sample volume - 10 µl/well Species - Human Application - The Calbiotech Secretory IgA (sIgA) ELISA Kit is intended for the quantitative measurement of sIgA in human stool or saliva. Principle - The Calbiotech Secretory IgA (sIgA) kit is a solid phase direct ELISA sandwich method. The standards, samples and controls are added into designated wells, coated with anti-sIgA monoclonal antibody, along with the incubation buffer. After a simple washing step, an anti-SIgA enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, a chromogenic substrate (TMB) is added into each well. Upon the addition of the substrate, the intensity of color developed is directly proportional to the concentration of sIgA in the samples. A standard curve is generated relating color intensity to the concentration of sIgA. Precautions - Not for use in diagnostic procedures.