HPTLC chromatography system AMD 2

for the food industryfor environmental analysisfor the pharmaceutical industry

The AMD 2 is a software-controlled HPTLC chamber for gradient development. It is used for difficult separation problems that cannot be solved by isocratic HPTLC. The separation of complex samples is a challenging task for every chromatographic system, particularly when the sample components span a wide polarity range. The AMD procedure offers an excellent solution as it allows stepwise gradient elution over increasing separation distances. As a result acids, bases, neutral, hydrophilic, and lipophilic substances can be separated in a single AMD run. This makes AMD suitable for a variety of applications. The technique is frequently used in lipid analysis and in routine analysis of drinking water. Pigment formulations with a complex composition, resins as well as additives of mineral oil products are other typical applications of AMD analysis. Key Features Multiple development Stepwise gradient elution over increasing separation distances Enhanced separation capacity HPTLC glass plates (20 x 10 cm), only Software-controlled with visionCATS The principle of the AMD procedure The HPTLC plate is developed repeatedly in the same direction Each successive run extends over a longer solvent migration distance than the one before Between runs, the solvent is completely removed from the developing chamber and the layer is dried under vacuum Each successive run uses a solvent of lower elution strength than that of the one used before. In this way, a stepwise elution gradient is formed. The combination of focusing effect and gradient elution results in extremely narrow bands. Their typical peak width is about 1 mm.