Automated spraying device for reagent transfer onto TLC/HPTLC plates
The Derivatizer is used for automated reagent transfer in the derivatization of thin-layer chromatograms and sets a new standard of reproducibility by employing a unique “micro droplet” spraying technology (patented). The Derivatizer ensures homogeneity and convenience in applying derivatization reagents, and offers other advantages as compared to manual spraying and immersion. The device is suitable for all common reagents. To meet the divergent physicochemical properties of the reagents, e.g. viscosity, four different color-coded nozzles are available, and the user can select from six spraying modes.
Key Features
Unsurpassed homogeneous reagent distribution
Environmentally friendly and safe handling through a closed system
Reproducible and user-independent results
Low reagent consumption (2-4 mL)
Hood for 20 x 10 cm and/or 20 x 20 cm plates
Intuitive handling and easy cleaning
Derivatization means another step in the process, consequently causing an increase in variance. Even if an experienced technician manually sprays a reagent, the relative standard deviation (RSD) of the measured values rises up to 12%. Using the Derivatizer, the RSD increases by less than 5%, thus providing very reproducible results, equal to those obtained with the Chromatogram Immersion Device, yet consuming considerably less reagent.
The following most common reagents have been tested and validated by the CAMAG laboratory for use with the Derivatizer:
Sulfuric acid reagent (10 % in methanol)
Anisaldehyde reagent
Natural product reagent
Polyethylene glycol solution
Iodine solution (0.5% in ethanol)