Dilution buffer reagent LowCross-Buffer®

laboratoryfor immunoanalysis

The formulation of LowCross-Buffer® helps to reduce interference in immunoassays. Thus quality of detection can be significantly improved. Nonspecific binding of the antibodies, negative effects of interfering substances and low or medium affinity cross-reactivities of the antibodies are minimized. In addition matrix effects, coming for example from blood sera or plasma specimen are reduced. That means that matrix effects no longer exacerbate detection of analytes. Minimization of all these negative effects upgrades the quality of the assay and improves the reliability of the results. LowCross-Buffer® is free of phosphate For general laboratory use LowCross-Buffer® is a newly developed sample buffer for immunoassays (e.g. ELISA, Western blotting, protein arrays and immuno-PCR). It helps to reduce interferences. Interferences are for example nonspecific binding, cross-reactivities and matrix effects. High affinity binding between antibody and antigen is not negatively effected, while unwanted binding of the antibody is inhibited. Most of the so called matrix effects are based on unwanted low or medium affinity binding of matrix components (e.g. serum proteins from human serum specimen) to analyte or antibody. The analyte can be masked by proteins or other components of the specimen. Thus the antibody can’t bind the analyte. Also antibodies can be masked by matrix components. LowCross-Buffer® reduces this masking of antibodies. LowCross-Buffer® is a dilution buffer for specimens (e.g. serum or plasma) and/or for detection antibodies. It depends on the interference.