Blocking buffer reagent 110 series

laboratory

The Blocking Solution saturates free binding sites on plastic surfaces, membranes or other protein binding surfaces. The Blocking Solution prevents nonspecific binding to surfaces. By incubating the surface with The Blocking Solution (e.g. microtiter plate or western blotting membrane), free spaces on the surface are saturated by the components of The Blocking Solution. This minimizes unwanted binding of the analyte or the detection antibody to the surface and so background can be avoided and sensitivity of the assay can be improved. The Blocking Solution is based on chemically modified and fragmented highly purified casein. The Blocking Solution is not comparable to other commercially available or home-made casein based solutions which are produced by just dissolving and filtration of casein. Benefits of The Blocking Solution Generally speaking, the blocking effectiveness depends on different factors. The more effective the blocker, the more reliable are the results of the assays (even with different specimens). It is important to achieve complete saturation of the surface without any gaps. There should be no exchange reaction between molecules of the blocking layer and the sample matrix. In addition any interaction of molecules in the blocking layer with the sample matrix should also be prevented. A good blocker can fill even very small gaps and can minimize such interactions. The Blocking Solution is the premium product of a new class of blockers developed by CANDOR Bioscience. These blockers carry chemical modifications which help to minimize interactions between the blocking layer and the sample matrix.