Blocking buffer reagent SmartBlock™

laboratory

SmartBlock™ is a modern blocker based on peptides. To further improve the blocking efficiency, peptides were chemically modified in a specific process. The result is a peptide solution with very good blocking characteristics. At the same time SmartBlock™ has a very good lot-to-lot consistency, up until now obtained only by synthetic blockers. SmartBlock™ sets standards in the development of economically priced assays with good reliability e.g. for diagnostics, pharmaceutical research, pharmaceutical or biotechnological process control, food diagnostics, environmental diagnostics or research. By incubating with SmartBlock™, free spaces on the surface (of e.g. microtiter plate or western blotting membrane) are saturated by the components of SmartBlock™. Thus it prevents unwanted binding of the analyte or the detection antibody to the surface. As a result background can be avoided. For many assays SmartBlock™ is an economical and useful alternative to BSA containing solutions. Problems caused by BSA included in the blocker such as BSA-interactions and cross reactivities can be eliminated by using SmartBlock™. For general laboratory use Benefits of SmartBlock™ The blocking efficiency depends on different aspects. The better the efficiency of the blocker, the more reliable are the results of the assays (even with different specimens). It is important to have a complete saturation of the surface with a dense and gap-free layer. Any interaction of molecules of the blocking layer with the sample matrix should also be prevented. A good blocker can even fill small gaps and should be designed to minimize interactions.