Enzyme reagent kit CVX™

RNase inhibitorfor researchfor drug development

CVX™ T7 ARCA mRNA Synthesis Kit is designed for in vitro ARCA-capped (m)RNA Synthesis by T7 RNA Polymerase. The cap structure is added to the mRNA by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA). ARCA is incorporated into mRNA exclusively in the correct orientation, generating capped mRNA that is more efficiently translated. By using a DNA template encoding a poly(A) tail, the CVX™ T7 ARCA mRNA Kit can be used to synthesize capped and tailed mRNAs. mRNAs synthesized with the CVX™ T7 ARCA mRNA Kit can subsequently be used for cell transfection, microinjection, in vitro translation and RNA vaccines. The kit contains sufficient reagents for 50 reactions of 20 μl each. A 20 μl reaction yields about 50 μg RNA with 1 μg of template added (1.5 kb RNA transcript). The T7 RNA Polymerase Enzyme mix includes Ribonuclease Inhibitor Protein to protect newly transcribed RNA from degradation by RNases. Advantages & Features High-Quality Kit: High enzymatic purity, specificity, and activity. Scaleable: Increase the reaction size to produce milligram amounts of RNA. Easy and time-saving protocol with minimal handling steps. Convenient: Rnase Inhibitor included. Applications Synthesis of ARCA-capped (m)RNA. Aantisense RNA and RNAi experiments. RNA vaccines. RNA structure and function studies. Advice Avoid freeze/thaw cycles. Safety Statements This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.