Solution reagent kit pOnebyOne™ I

growth factorfor PCR

pOnebyOne™ are extremely efficient, accurate and flexible Bicistronic Mammalian Expression Kits that contains an expression cassette based in 2A sequence Breakthrough technology. The expression cassette of pOnebyOne™ I (with Neomycin resistance) incorporates the cytomegalovirus early promoter that precedes 2A sequence in frame with truncated nerve growth factor receptor (ΔNGFR). ΔNGFR is a complete solution to select positive clones. It could be visualized by cytometry using specific antibody labelled with FITC or similar and also, it could be enriched from negative clones with magnetic beads bearing anti-ΔNGFR antibody. Advantages & Features Complete solution: a directional cloning vector to clone and produce a protein of interest. Breakthrough technology: based in 2A sequence simultaneous expression of two proteins in mammalian cells (a protein of interest and reporter). Highly efficient: cloning system tested with up to 4 kb inserts. Time-saving cloning process: linearized vector ready-to-mix with your PCR fragment. Easy-to-use: facilitates the selection of positive cells expressing the recombinant gene of interest. Cost avoidance: avoids the use of restriction enzymes. Accurate: proven performance for most common cell lines. Flexible: allows transfection in difficult cell lines. Really low experimental background: lower than 1%. Convenient: available with different resistance marker cassettes. Directional cloning: of PCR with the gene of interest. Reporter checking: stoichiometric amount of your protein of interest and a reporter protein. Risk-free: product covered by our Quality 100% Guarantee.