Solution reagent kit FastCONTROL™

for researchproteinalkaline phosphatase

FastCONTROL™ Dual Reporter Plasmid Controls are fast, convenient and flexible target cell transfection control ideal for high co-expression of two reporter genes drive for ubiquitous, strong and constitutive promoters. FastCONTROL™ p2V-SEAP/ eGFP-I Dual Reporter vector contains cytomegalovirus promoter (Pcmv) to drive the expression of both secreted embryonic alkaline phosphatase (SEAP) and green fluorescent protein (GFP). GFP from Aequoria victoria has been optimized for brighter and higher expression in mammalian cells; it could be visualized by cytometry or microscopy (Excitation wavelength maximum=488 nm/ Emission wavelength maximum=507 nm). Advantages & Features Fast: available combination of 2 cell location markers in one vector. Convenient: available in Lentiviral, Retroviral or non-viral vectors, for immediate in vivo and in vitro expression. Compatible with transient or stable transfections. Flexible: available with different mammalian resistance markers and Dual Reporter genes combination for different cell location. Risk-free: product covered by our Quality 100% Guarantee. Includes: – 15 µL FastCONTROL™ Dual Reporter (1 µg/µL) Plus Version – Includes: – 15 µL FastCONTROL™ Dual Reporter (1 µg/µL) – 0.2 mL CANFAST™ Transfection Reagent (1 mg/mL) Applications Control for assessing the efficiency of transfection in mammalian cells. Targering of different cell locations. Obtention of cell lines with reporter proteins. Quality Control The quantity and quality of purified DNA attend to: – Ratio 260/ 280 (1.8 – 2.0). – Agarose gel Electrophoresis. – Digestion with Restriction Endonucleases.