pASSEMBLE™ Ecotropic Retroviral Packaging System includes a unique packaging vector with gag, pol and env from different viruses confering a tropism in the cell to be infected. Ecotropic Packaging System includes a packaging vector with gp70 envelope genes from Moloney murine leukaemia virus (MoMLV). The gp70 envelope glycoproteins of ecotropic MoMLV viruses bind to receptors that occur only on mouse and rat cells and on interspecies hybrid cells that contain mouse chromosome 5. In murine cells, the entry of this ecotropic virus is mediated by mCAT-1 receptor. Higher efficiency of transduction is obtained by infection of cells previously transfected with mCAT-1 expression vector.
Advantages & Features
Higher efficiency of transduction.
High titer.
Ecotropic vector.
Risk-free: product covered by our Quality 100% Guarantee.
Specifications
Tropism: Mouse and rat cells.
Includes
ME0040
– 20 μL pASSEMBLE™ Ecotropic Retroviral Packaging Vector (50 ng/μL)
ME0040-Plus
-20 μL pASSEMBLE™ Ecotropic Retroviral Packaging Vector (50 ng/µL)
-0.2 mL CANFAST™ Transfection Reagent
-20 μL eGFP Retroviral Transfer Control Vector (50 ng/µL)
-20 μL mCAT-1 Expression Vector (50 ng/µL)
Applications
Packaging of Retroviral Transfer vectors.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.