Enzyme reagent kit Horse-Power™

solutionTaq DNA polymerasefor PCR

Horse-Power™ Taq DNA Polymerase is a pure, versatile and thermostable recombinant enzyme produced in an E. coli strain, which carries the cloned pol gene from Thermus aquaticus. The enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Advantages & Features Highest purity: greater than 98% confirmed by SDS-PAGE. Highest quality: high activity, specificity, thermostability and performance in PCR. Highly efficient: reactivation buffer improved. Thermostable: half-life at 94 ºC is 40 minutes. Adds extra nucleotides: preferentially adenine, without template at 3´ends leaving 3´overhangs PCR fragments. Incorporates modified nucleotides: biotinylated, fluorescently labelled, etc. Molecular Weight: 94 kDa. Convenient: available in different concentrations, sizes and solutions. Complete solution: includes MgCl2. Risk-free: product covered by our Quality 100% Guarantee. Specifications Assay conditions: 25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA. Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C. Includes Concentration: 5 U/μL – Includes for 500 U: – 100 μL Horse-Power™ Taq Polymerase (5 U/μL) – 25 mM MgCl2 (1.5 mL) – 1.5 mL Buffer (10x) Concentration: 1 U/μL – Includes for 500 U: – 500 μL Horse-Power™ Taq Polymerase (1 U/μL) – 25 mM MgCl2 (1.5 mL) – 1.5 mL Buffer (10x)