Enzyme reagent kit PickMutant™

DNA polymerasefor researchprotein

PickMutant™ is a reliable, robust and highly efficient PCR-based Mutagenesis Kit. Extremely easy-to-use, the kit allows creating single or multiple point mutations, deletions or insertions using a rapid and easy protocol. All these mutation could be obtained by PCR using a FastPANGEA™ High Fidelity DNA Polymerase and well-designed Mutagenesis primers. The assembled mutagenic PCR fragments is cloned into Canvax™ pSpark® Cloning vector, specially designed to clone blunt PCR fragments with high efficiency or into other vector designing, in this case, an additional specific vector primer pair. Advantages & Features Highly Effective point mutations (single or multiple), deletions or insertions. Easy and fast protocol: it takes less than 3 hours in one step procedure. Cost avoidance: compatible with any bacterial strains or primers. Versatile: compatible with any Cloning vector. Efficient: includes highly efficient pSpark® to clone blunted fragments. Robust: simultaneous assemble and clone of PCR fragments. Risk-free: product covered by our Quality 100% Guarantee. Includes Includes for 15 rxn: – 150 μL Proofreading DNA Polymerase MasterMix (2x) – 300 U Glue Enzyme (10 U/μL) – 40 μL Glue Enzyme Buffer (10x) – 5 μL Insert Control DNA – 15 μL pSpark® I (20 ng/μL) Applications Site-directed Mutagenesis. Study protein function. Identify enzyme active sites. Design new proteins. Quality Control Tested using the insert control DNA provided. Safety Statements This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO).