qMAXSen™ Green qPCR MasterMix 2x (w/o ROX™), offers exceptional Sensitivity, Specificity & Performance for real-time PCR using a Green fluorescent dye. Supplied as stabilized 2x reaction mixture, is a convenient and easy, ready-to-use premix to perform qPCR using an analogue fluorescent dye to SYBR® Green. The optimized MasterMix includes all the components required for running PCR reactions, except PCR Primers and template.
Available with the option of ROX™ as the internal passive reference dye. It provides an internal reference to which the reporter-dye signal can be normalized during data analysis.
Advantages & Features
Exceptional Sensitivity: for increased limit of detection.
High Specificity: due its Hot-Start Antibody technology.
Safe: minimize contamination risk due its optimized protocol that reduces pipetting steps.
Great signal-to-noise ratio.
Versatile: compatible with most used real-time PCR instruments.
Accurate Quantification of a variety of gene targets.
Risk-free: product covered by our Quality 100% Guarantee.
Specifications
Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus.
Includes
Includes for 250 reactions:
– 2 x 1.25 mL qMAXSen™ Green qPCR MasterMix 2x (w/o ROX)
The MasterMix includes 2x mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, Reference dye & Proprietary Buffer Components.
Applications
Detection and quantification of DNA and cDNA targets.
Gene expression.
Low copy detection.
High throughput applications.
qPCR for post reverse transcription step.
Quality Control
Functionally tested in Real Time PCR on Applied Biosystems StepOne® Real-Time PCR System.