DNA polymerase reagent kit HotBegan™

antibodyTaq DNA polymerasefor PCR

HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products. Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable. Advantages & Features High specificity: minimizes unspecific amplification. Efficient: prevents or minimizes primer-dimer and nonspecific products. Great sensitivity: amplifies from femptograms of DNA targets. Inactive: at Room Temperature. Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments. Powerful: amplification of targets up to 5 kb. Convenient: available in kits and MasterMix solution. Risk-free: product covered by our Quality 100% Guarantee. Specifications Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C. Includes Includes for 500 U: – 100 μL HotBegan™ Hot Start Taq DNA Polymerase (5 U/μL) – 25 mM MgCl2 (1.5 mL) – 1.5 mL Buffer (10x) Quality Control Functionally tested in qPCR. None detected bacterial DNA (by qPCR).