CleanEasy™ Agarose Purification Kit provides an accurate, rapid and highly efficient method to extract DNA from agarose gels. The kit uses HigherPurity™ breakthrough technology based in solubilisation and binding of DNA to a silica membrane in presence of chaotropic salts. Comfortable CleanEasy™ MiniSpin Columns contains an exclusive membrane that allows binding a unique DNA fragment, previously excised from agarose gel.
Advantages & Features
Extremely fast and easy procedure: it takes 5 minutes to results with minimal handling steps.
Reproducible yields: higher than 80% DNA Recovery (0.7 – 1% agarose) and reproducible yields of pure DNA.
Versatile: compatible with a wide spectrum of size fragments, suitable since 100 bp up or any kind of agarose and gel buffer systems.
Pure genomic DNA: ready-to-use in all Molecular Biology applications.
Risk-free: product covered by our Quality 100% Guarantee.
Includes
Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes (2 mL)
– 60 mL QG Buffer
– 11.25 mL PE Buffer
– 10 mL EB Buffer
Applications
– Purification of DNA fragments (obtained by PCR or digestion with restriction enzymes) from agarose gels.
– Purified DNA suitable for all common Molecular Biology applications, such as:
PCR.
Cloning.
DNA sequencing.
Southern blot analysis.
Quality Control
Tested in the purification of a 0.5 kb DNA fragment excised from 2% agarose gel.
The purified band is analysed in agarose gel electrophoresis and quantify by spectrophotometry Ratio 260/ 280 (1.8-2).
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO).