Enzyme reagent kit HigherPurity™

for molecular biologygenomic DNAbacteria

HigherPurity™ Bacterial Genomic DNA Isolation Kit provides an accurate, easy-to use and rapid method to isolate high quality DNA from both Gram negative and Gram positive bacteria. The kit uses HigherPurity™ breakthrough technology based in the ability to bind silica in the presence of high concentrations of chaotropic salts as guanidinium thiocyanate. The extraction process uses comfortable CleanEasy™ MiniSpin Columns and includes an initial cell-wall lysis step with the appropriate enzyme to ensure efficient cell lysis and DNA release from the cell. Advantages & Features Highly efficient: yields up to 24 mg. Really fast and easy procedure: it takes 78 minutes to get results with minimal handling steps. Safe: avoids phenol/chloroform extraction. Convenient: ideal for bacterial DNA isolation from cell pellets after culturing. Risk-free: product covered by our Quality 100% Guarantee. Includes Includes for 50 rxn: – 50 CleanEasy™ MiniSpin Columns – 50 Collection tubes – 15 mL BR-1 Buffer – 20 mL BLU Buffer – 30 mL WB1 Buffer – 30 mL WB2 Buffer – 15 mL EB Buffer – 30 mg Proteinase K – 25 mg Lysozyme Applications Purified DNA suitable for all common Molecular Biology applications, such as: Digestion with restriction enzymes. Automated sequencing. PCR template. Southern Blots. Quality Control Tested on a lot-to-lot basis by isolating total DNA from E. coli. Tested for the isolation of any Plasmid DNA from transformed E.coli. The Quality of Purified DNA is analysed by: – Ratio 260/ 280 (1.8-2). – Agarose Gel Electrophoresis. – Digestion with Restriction Endonucleases.