Tris-Glycine Buffer (TG) is a high quality and convenient running buffer in native (non-denaturing) homogeneous and gradient poly-acrylamide gel electrophoresis (PAGE) of proteins. Tris-glycine gels resolve proteins by size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front.
It is also used to make Tris-glycine/20% methanol Western transfer buffer, which is the most frequently used protein transfer buffer for wet blot transfers.
Advantages & Features
Reliable: rigorous quality control standards to guarantee lot-to-lot consistency.
High Quality: free of DNAse, RNase or protease contamination.
Proven performance: for protein electrophoresis.
Consistency guaranteed.
Risk-free: product covered by our Quality 100% Guarantee.
Specifications
Chemicals: Analytical grade.
Composition: 0.025 M Tris, 0.192 M glycine.
pH: 8.3 ± 0.2 at 25 °C.
Includes
Tablets format – Includes for 1 pouch:
– Exactly pre-weighed pouchs (1,000 mL/pouch)
Aqueous solution format – Includes for 1 unit:
– 1,000 mL of Tris-Glycine Buffer
Applications
Protein Electrophoresis.
Denatured protein Electrophoresis.
Polyacrylamide gel Electrophoresis.
Western Blotting.
Quality Control
Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
Recommendations: Store the product in a dry place.
Storage, Shipping & Guarantee
Shipped at: Room temperature.
Storage: Room temperature.
Shelf Life: 24 months from the date of purchase, if it is properly stored.