Ampicillin reagent kit pSpark® III

DNA ligasefor PCR

For highly Efficient, Accurate & Easy Cloning with Ampicillin and Kanamycin resistance Product Overview: pSpark® III is a highly efficient, accurate and easy-to-use DNA Cloning System that combines Ampicillin and Kanamycin resistance. Ideal for cloning PCR products amplified from any plasmid vector without the need to gel-purify bands to eliminate the background due to the template vector used for PCR. Advantages & Features Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions. Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation. Powerful: obtain 5-fold more positive colonies using 10x less DNA insert. Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding «suicide» strategies from toxic genes. High stability: eliminates cloning bias or pitfalls. Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers. Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert. High cost-saving: reduces your cloning costs as no expensive phosphorylated primers are needed. Eliminates positive selection vector. Risk-free: product covered by our Quality 100% Guarantee. Includes Includes for 20 rxn: – 20 µL pSpark® III (20 ng/µL) – 200 µL T4 DNA Ligase Buffer (5x) – 150 µL PEG 6000 (10x) – 5 µL Insert Control 1 kb (20 ng/µL) Applications Cloning directly from PCR using plasmid cloned genes as template. Unpurified PCR cloning. Cloning of high fidelity PCR amplified products. Production of ssDNA.