Solution reagent kit HotBegan™

antibodyTaq DNA polymerasefor real-time PCR

HotBegan™ Hot Start Taq DNA Polymerase MasterMix (2x) an optimized ready-to-use solution containing HotBegan™ Taq DNAPolymerase (hot start performance), dNTPs, MgCl2 and Stabilizers. It is inactive at Room Temperature and includes all material needed except Template and Primers. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products. Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable. Advantages & Features Time-saving solution: ready-to-use MasterMix. High specificity: minimizes unspecific amplification. Efficient: prevents or minimizes primer-dimer and nonspecific products. Great sensitivity: amplifies from femptograms of DNA targets. Inactive: at Room Temperature. Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments. Powerful: amplification of targets up to 5 kb. Convenient: available in kits and MasterMix solution. Risk-free: product covered by our Quality 100% Guarantee. Specifications Unit definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.