The FluoroCore™ M2 is a dedicated ELISPOT reader that additionally excels at high-throughput PBMC counting, and supports several assays that are standard in immune monitoring laboratories. Live cell counting is the rate-limiting step for testing multiple PBMC samples in ELISPOT assays, and the accuracy of this cell counting represents the largest variable in ELISPOT assay results. The FluoroCore™ is equipped with optics and an extra fluorescent channel which, along with CTL’s dedicated LDA Cell Counting Suite, can count dozens of PBMC samples within minutes, including the documentation of viability, and the calculation of resuspension volumes.
The live cell counting ability, along with the TVA Software, renders the FluoroCore™ M2 as a high-throughput reader for cell-mediated cytotoxicity assays. Its fluorescent channel enables the Analyzer to conduct single-color FluoroSpot analysis when such is preferable over ELISPOT, e.g., for the precise quantification of per-cell secreted analyte via the HDR imaging feature, and FCS standard data output, all of which are supported by the FluoroX™ Suite. Enabled by the BioSpot™ Software, this instrument permits high-throughput reading of viral neutralization and infectivity assays in 96- or 384-well formats. The instrument comes with features supporting work in a regulated environment, and is high-throughput capable, handling up to 400 plates per run when equipped with our automated plate loader.
Specifications
Applications
Single-Color ELISPOT
Single-Color FluoroSpot
Single-Color BioSpot
FRNT / FRA
PRNT / Viral Titer Assays
Bacterial/Yeast/Viral Colony Counting
Live PBMC Cell Counting