Memory B cells exist in a quiescent state in the absence of recent antigen encounter and importantly do not secrete their individual BCR as soluble antibody. Therefore, polyclonal stimulation of resting memory B cell is required for their measurement using ELISPOT or FluoroSpot (collectively ImmunoSpot® ). Mouse B-Poly-S™ promotes polyclonal stimulation and differentiation of B cells into antibody-secreting cells (ASC) capable of producing IgA, IgG or IgM.
Details
CTL Mouse B-Poly-S™ is a stock solution containing Resiquimod and recombinant Mouse IL-2 and is capable of polyclonal stimulation and differentiation of B cells into antibody-secreting cells (ASC) producing IgA, IgG or IgM.