Stain reagent kit MicroStacker™

staining solutionenzymeantibody

MicroStacker™ Polymer Detection System Universal is a biotin-free, polymeric horseradish peroxidase (HRP)- secondary antibody conjugate system for the detection of mouse and rabbit primary antibody on formalin-fixed, paraffin-embedded (FFPE) tissues in an immunohistochemistry (IHC) procedure. The innovative MicroStacker™ technology allows well-controlled layered stacking of antibodies and peroxidase enzymes on a micro-polymer scaffold. This technique results in a compact polymeric structure that easily penetrates to all cellular compartments, which provides superior sensitivity compared to other conventional HRP polymers with bulky dextran backbones. Plus, the system utilizes F(ab’) fragments of IgG secondary antibody instead of the whole IgG, which avoids the background caused by non-specific binding of whole IgG to endogenous Fc receptors. Reagent Composition Code: SD3102(5 Components) Peroxidase Blocking Reagents MicroStacker™ Anti-Mouse/Rabbit HRP-Polymer 20*DAB Chromogen DAB Substrate Buffer Hematoxylin Also, 3 Components & 4 Components kits are available. Type: Ready-to-Use(RTU) Specifications: 200T/kit, 300T/kit, 1000T/kit Recommended Protocol Deparaffination. Peroxidase Block: Block for 5mins with Peroxidase Blocking Reagents. Rinse 2 times for 5mins each. Antigen Retrieval Protocol: Refer to the primary antibody datasheet. Primary Antibody: Apply 2 drops (100ul) or as much as needed of ready-to-use primary antibody. Incubate for 30-60mins at RT. Rinse 2 times. Detection Polymer: Apply 2 drops (100ul) or as much as needed of anti-Mouse/Rabbit HRP-Polymer. Incubate for 30mins at RT. Rinse 2 times.