EBER probe (ISH) can detect Epstein-Barr virus infection in situ combined with cell line marker immunofluorescence to do double labeling, and show positive cell type of virus. CD3 is commonly used to label T lymphocytes. CD20 is used to label B lymphocytes. Double staining can determine the classification and character of lymphoma at molecular and protein levels on the same section like diffuse large B cell lymphoma or anaplastic T cell lymphoma. This method can improve diagnostic accuracy.