Antibody test kit

COVID-19IgGIgM

This kit is a colloidal gold immunochromatographic testing product for the qualitative determination of IgM and IgG antibodies against novel coronavirus (COVID-19) in human serum, plasma, whole blood or fingertip blood. Antibody-antigen sandwich one-step assay has been used in this detection. For the test line (M line and G line), colloidal gold conjugated COVID-19 antigen is used as a detector and coated on the conjugate pad, and mouse anti-human IgM antibody and mouse anti-human IgG antibody are used as the two captures and coated separately on nitrocellulose membrane. For the quality control line (C line), colloidal gold conjugated rabbit IgG is used as a detector and coated on the conjugate pad and goat anti-rabbit IgG antibody is used as the capture and coated on nitrocellulose membrane. When a sample is loaded on the conjugate pad, the IgM or IgG antibody against the virus antigen in the sample specifically binds to the virus antigen that is conjugated with gold, forms an IgM or IgG antibody-antigen gold complex, then the complex migrates on the nitrocellulose membrane and is captured by mouse anti-human IgM or IgG antibody and immobilized on the nitrocellulose membrane, and then red bands appear on the detection line (M line and G line). The gold labeled rabbit IgG is captured by goat anti rabbit IgG antibody and forms a red quality control line (C line) on the nitrocellulose membrane at the same time. Sample requirements 1. The sample can be whole blood or serum, plasma. The anticoagulants used for plasma collection could be sodium citrate, EDTA.2Na or heparin. Venous blood or fingertip blood should be collected in sterile condition.