Solution 6 – VB-48™•PI is a staining reagent containing a thiol-reactive fluorescent probe, VitaBright-48™ and propidium iodide (PI). Solution 6 – VB-48™•PI enables the user to detect changes in cell vitality.
Solution 6 – VB-48™•PI contains two different dyes: PI staining nonviable cells only and VB-48™ which stains cells in an intensity-dependent manner reflecting their redox status. A high fluorescence intensity of a particular cell indicates that the cell has a high level of reduced thiols. VitaBright-48™ can be used as a marker for detection of mid- to late-stage apoptosis in mammalian cells as high levels of reduced thiols within any given cell will result in a high fluorescence intensity of VB-48™. Please refer to the specific Application Notes for detailed instructions.
The solution is for research use only (RUO) and is not for diagnostic or therapeutic uses. Please refer to the documents below for further details. Order the product in our Webshop.
Use for
Vitality Assay with the NucleoCounter® NC-250™.
Package Insert
Certificates
SDS
Cell Assays