Medium reagent CO262

for bacterial isolationfor urine samplesveterinary laboratory

Polymyxin E (colistin) and B are increasingly used as antimicrobials in the treatment of multi-drug resistant bacterial infections. Polymyxin resistance, although intrinsic in Gram-positive and some Gram-negative species (Proteus, Morganella, Serratia), is now a problem in a number of other pathogens(Acinetobacter baumannii, Pseudomonas aeruginosa, Escherichia coli, Salmonella enterica, Klebsiella pneumoniae).* Resistance arises due to mutations/insertions in genes involved in LPS biosynthesis (lpx, pmrA/B, mgrB, phoP/Q) and/or the acquisition of phosphoethanolomine transferases (PEtN).Of great concern is the recently described plasmid-encoded PEtN, MCR-1, now found worldwide in a range of animal, human and environmental bacterial isolates.* CHROMagar™ COL-APSE is a sensitive and specific medium for the growth of Colistin resistant bacterial pathogens with a lower limit of detection of 10 CFU/mL. This new chromogenic medium may be useful as a primary isolation medium in the surveillance and recovery of Colistin resistant bacteria from complex human, veterinary and environmental samples especially those with plasmid mediated MCR-1 or novel mechanisms of polymyxin resistance.* * Novel Chromogenic Culture Media (CHROMagar™ COL-APSE) for the Isolation and Differentiation of Colistin Resistant Gram-negative Pathogens. Muhd Haziq F Abdul Momin, David W Wareham. ECCMID 2017. Intended Use: CHROMagar™ COL-APSE is a selective and differential chromogenic culture medium, intended for use in the qualitative direct detection of gastrointestinal colonization with colistin-resistant Gram-negative bacteria (COL-R) to aid in the prevention