Solution reagent CNGS-0 series

NGSliquid

Since its introduction in 2005, Next Generation Sequencing (NGS) opened many doors in the fields of translational genomics and molecular diagnostics by massive parallel decoding of DNA or RNA fragments. To generate high quality NGS data, preparation of pure DNA or RNA of a specific length is one of the key process steps. We offer our CleanNGS for library cleanup and size selection to make this process simple and reliable. Our special buffer formula ensures optimal size selection for NGS libaries and the high quality magnetic beads allow faster separations and better RNA/DNA recovery. CleanNGS is produced RNase free, which makes it an ideal solution for all downstream RNA or DNA NGS experiments. CleanNGS is suited for cleanup in between library prepping steps, after library prepping and for size selection in next-generation sequencing or sanger sequencing. It is also a highly efficient cleanup method to use before other techniques such as PCR, cloning or CRISPR-Cas9 to improve the quality of your results. Our purification reagent provides maximum flexibility allowing for left, right or double-sided size selection by easily adjusting the sample to CleanNGS volume ratio(s). Based on CleanNA’s proprietary chemistry CleanNGS removes, salts, primers, primer-dimers and dNTPs, while DNA and/or RNA fragments are selectively bound to the magnetic particles based on their size. Purified DNA and RNA is eluted of the magnetic particles using water or a low salt buffer and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Hamilton, Beckman, Agilent, Caliper, Perkin Elmer, Tecan and Eppendorf)