Antibody F.QUANT Titer

serumplasmafor biological samples

F.QUANT, powered by PAIA, is a bead- and plate-based fluorescence assay that measures the concentration of target antibodies in biological samples (e.g. serum, plasma and culture supernatant). The kit contains a bead-filled 384-well plate and a fluorescent reagent mix for fast and direct titer measurement, enabling reliable identification of high-producing cell lines for manufacturing biotherapeutics and high-throughput antibody discovery workflows. CYTENA offers two different F.QUANT Titer assay kits. The F.QUANT Fc Titer assay is specific for Fc in IgG and other proteins containing the Fc part of IgG, such as Fc fusion proteins and bispecifics, while the F.QUANT Fab Titer assay recognizes human Fab fragments. Key Features & Benefits Find out how our product can enrich your research Direct titer quantification from supernatant Mix your sample with the F.QUANT Titer mix in the F.QUANT Titer plate for direct quantification. No cell removal or wash necessary Pipette the supernatant directly into the F.QUANT Titer plate without washing steps needed, given that residual cells do not affect the results. Optimized for clone screening in CLD Use our optimized protocols for monoclonal antibodies. Mix, shake and read workflow Easily measure the titer of your samples in just a few simple steps. Simple automation on liquid handlers Set up the F.QUANT Titer assay easily in your automated CLD workflows. Assays require as little as 5 µL Take out a small volume of cell supernatant for measurements and continue with culturing. Kit contents Each kit includes 384-well F.QUANT Titer Plates, with capture beads in each well, and the corresponding fluorescent F.QUANT Titer Mix.