Growth medium reagent Kit 2.0

for cell culturefor researchliquid

The Natural Killer Cells Induction Culture Kit 2.0 is a GMP-grade in vitro induction and expansion culture kit.The product contains NK activator ,NK stimulator and N500 Serum-Free Medium. This product is of stable batch-to-batch quality and is used to expand and culture NK cells from human peripher al blood mononuclear cells (PBMCs)without feed cells. Product characteristic High Safety: The kit is chemically defined, xeno-free, animal component-free, serum-free and is designed for use without feeder cells. Higher yield of NK cells with higher purity can be obtained with our kit. Convenient and Time-saving: Coating is not required during the process, the kit is ready to use. Viability During 14 days of culture ,the viability of cells maintains above 90%. CD3-CD56+Cell Proportion After 14 days of culture, the proportion of NK cells(CD3-CD56+)can reach to 60%~95%,complying with the requirements of experimental and human transfusion. Cell Proliferation After 14 days of culture，4 billion of cells can be harvested per 1L kit of NK2.0. NK Cell Cytotoxicity After 14 days of culture, as measured by the lactate dehydrogenase (LDH) release assay, The cytotoxicity of harvested NK cells to K562 cells is 40%–80% in vitro, and K562 cells is 5:1.