Medium reagent Biosci™

for scientific researchfor cell culturefor flow cytometry

Human Lymphocyte Separation Medium is a sterile, endotoxin tested and ready-to-use solution. This product contains polysucrose, which accelerates erythrocyte precipitation, helps to improve the separation result of lymphocytes. The lymphocytes isolated are available for the cell culture and cytotoxicity assays. This product is mainly used for the separation of human peripheral blood lymphocytes, but also for the separation of most mammalian mononuclear cells. Principle of the Procedure The density of mononuclear cells (lymphocytes and monocytes) in peripheral blood is between 1.075-1.090g/ml, which is different from that of red blood cells, multinuclear leucocytes and platelets.The densities of red blood cells and granulocytes are relatively large(about 1.092g/ml) and the density of platelets is between 1.030~1.035g/ml.Therefore, Human Lymphocyte Separation Medium is used to generate a certain degree of density gradient, and the diluted whole blood is smoothly paved on the separation medium. After centrifugation, the red blood cells and granulocytes sink to the bottom of the tube due to their large density; Since their densities are less than or equal to the density of separation medium, lymphocytes and monocytesare found at the surface of separation medium. There may also be a small amount of cells suspended in the separation medium. By sucking the cells on the surface of separation medium, mononuclear cells can be separated from peripheral blood. Sample Type Human peripheral blood lymphocytes; Most mammalian mononuclear cells. Intended Use Separation of human peripheral blood lymphocytes; Separation of most mammalian mononuclear cells.