Medium reagent Biosci™

for cell culturefor scientific researchfor flow cytometry

The density of mononuclear cells (lymphocytes and monocytes) in peripheral blood is between 1.075-1.090g/ml, which is different from that of red blood cells, multinuclear leucocytes and platelets.The densities of red blood cells and granulocytes are relatively large(about 1.092g/ml) and the density of platelets is between 1.030~1.035g/ml.Therefore, Human Lymphocyte Separation Medium is used to generate a certain degree of density gradient, and the diluted whole blood is smoothly paved on the separation medium. After centrifugation, the red blood cells and granulocytes sink to the bottom of the tube due to their large density; Since their densities are less than or equal to the density of separation medium, lymphocytes and monocytesare found at the surface of separation medium. There may also be a small amount of cells suspended in the separation medium. By sucking the cells on the surface of separation medium, mononuclear cells can be separated from peripheral blood. This series of products has a series of advantages such as fast and simple use, no cytotoxicity, high Cell yield and good vitality. Troubleshooting The optimum separation temperature is (20±5)℃, beyond which the separation effect will be affected. Pre-warm separation medium to room temperature (20±5℃) and shake gently before use. Avoid using the separation medium directly from refrigerator. For The Separation Medium, The isotonic medium (PBS or 0.9% NaCl) used to dilute anticoagulated blood should be sterile, and can be replaced by RPMI-1640 medium. 1:1 blood dilution can reduce the coagulation of erythrocytes and increase the amount of lymphocytes harvested.