Loading buffer reagent SuperCut™ XhoⅠ

enzymefor researchfor molecular biology

A.General Description SuperCut™ XhoⅠ Cat. No.: 8052441 Restriction Enzyme Site 5'…C↓T C G A G…3' 3'…G A G C T↑C…5' Isoschizomers*:PaeR7I，TliI，BssHI，Sfr274I，SlaI，StrI *Isoschizomers may have different methylation sensitivities. SuperCut™ quick enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products, or genomic DNA. SuperCut™ quick enzymes have the following characteristics: digestion can be completed within 5~15 minutes; sharing a digestion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or complex template digestion. In addition, the dephosphorylation and ligation re-agent of BiosciTM is 100% active in SuperCut™ Buffer, sup-porting one-tube reaction and improving the experience of "digestion-modification-ligation". B. Recommended Reaction Conditions 1× SuperCut™ Buffer； Incubate at 37℃； Refer to "Protocol for Fast DNA Digestion" for reaction setup. C. Heat Inactivation 1. Incubation at 80℃ for 20 minutes. 2. Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction. D. Product Composition Components Amount SuperCut™ XhoⅠ (10 units/μl) 5000 units SuperCut™ 10× Buffer 3×1 ml 6× Gel Loading Buffer 1 ml E. Storage Condition Storage at -20℃ and validity for 24 months. Shipped on Blue Ice.