Hematoxylin-Eosin Staining Solution
A. Intended Use
Hematoxylin-Eosin Staining Solution is mainly used for staining of tissues and cells.
B. Principle/Summary
The most commonly used staining system is called H&E (Hematoxylin and Eosin). H&E mainly contains the two dyes hematoxylin and eosin. Hematoxylin are often employed to color the nuclei of cells blue. Eosin is an acidic dye, it stains cytoplasm red or pink. By hematoxylin eosin staining of cell smear or tissue section, the nuclei will be stained blue purple the cytoplasm, muscle fiber, collagen fiber, etc. will show different degrees of red, and the red cells will show orange red. Biosci™ Hematoxylin-Eosin Staining Solution uses the optimized combination reagent, which can make the staining tissue structure-clear and coloring-sustained.
C. Main Compositions
Biosci™ Hematoxylin-Eosin Staining Solution is composed of Hematoxylin, Hematoxylin Buffer, Eosin, Differentiation Solution and Bluing Solution.
Component No. Component Name Specification Quantity
4960311-1 Hematoxylin 500mL/bottle 1 bottle
4960311-2 Hematoxylin Buffer 500mL/bottle 1 bottle
4960311-3 Eosin 500mL/bottle 1 bottle
4960311-4 Differentiation Solution 500mL/bottle 1 bottle
4960311-5 Bluing Solution 500mL/bottle 1 bottle
D. Storage and Stability
Storage at 10℃ - 30℃, valid for 12 months.
E. Sample Requirements
Smears and sections must be fully fixed, and paraffin sections must be fully deparaffinized.