Medium reagent SuperCulture™ N500

for researchlactate dehydrogenase

The SuperCulture™ N500 serum free medium for NK cells is a special medium for in vitro culture of NK cells developed by Dakewe, and is recommended to be used with the NK cell activator (Cat#: 2.C.NK01) and the NK cell stimulator (Cat#: 2.C.NK04), or can be used through other in vitro culture schemes for NK cells. This product is a chemically defined medium, all the compositions of which are produced from medicinal/cell culture grade materials. This product contains no animal component or serum or any other protein component except albumin, transferrin and insulin and can effectively avoid the influence of serum qualitative variation, serum components and exogenous components on experimental research. The optimized formula of this product can significantly improve the proportion, the viability and the killing rate of NK cells in cells cultured in vitro. Product characteristic High Safety: The kit is chemically defined, xeno-free, animal component-free, serum-free and is designed for use without feeder cells. Higher yield of NK cells with higher purity can be obtained with our kit. Convenient and Time-saving: Coating is not required during the process, the kit is ready to use. Viability During 14 days of culture, the viability of cells maintains above 90%. CD3-CD56+Cell Proportion After 14 days of culture, the proportion of NK cells(CD3-CD56+)can reach to 60%~95%,complying with the requirements of experimental and human transfusion. Cell Proliferation After 14 days of culture，4 billion of cells can be harvested per 1L kit of NK2.0. NK Cell Cytotoxicity After 14 days of culture, as measured by the lactate dehydrogenase (LDH) release assay,