Research detection kit 2110002

for growth factorsFor TNFαELISA

Shenzhen Dakewe Bio-engineering Co., Ltd. independently developed and mass-produced cytokine ELISA kits with high efficiency, time saving, high quality and low price, etc. So far, it has completed the development of a total of 83 products including Human, Mouse and Rat and other common targets. Cytokine types include interleukin, interferon, growth factor, colony-stimulating factor, tumor necrosis factor, chemokine, etc. Some products achieve the reduction method, the shortest operation time is only 2 hours, which can greatly reduce the labor intensity of the operator and shorten the operation time. ELISA Troubleshooting Guide Problem - Possibility - Solution (1) Improper and inefficient washing - (1) correct Washing (2) Reagents have expired - (2) Do not use the expired reagents (3) Incorrect dilutions, conjugate concentration was too low - (3) Repeat test using correct dilutions, check with the recommendations of the antibody manufacturer Very weak signal (4) Inefficient incubation time - (4) Repeat ELISA, follow the protocol carefully for each step’ s incubation time (5) Incorrect storage of reagents - (5) Store reagents in the correct temperature, avoid freeze and thaw, avoid using the “frost free” freezer (6) Wrong filter in ELISA reader was used - (6) Use the correct wavelength setting (1) Improper and inefficient washing - (1) correct Washing (2) Poor mixing of samples - (2) Mix samples and reagents gently and warm to room temperature (3) Plates not clean - (3) Plates should be wiped on bottom before measuring absorbance (4) Wrong filter in ELISA reader was used - (4) Use the correct wavelength setting