The Rat IL-4 ELISA kit is to be used for the in-vitro quantitative determination of IL-4 in rat serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant rat IL-4 .
This kit has been configured for research use only and is not to be used in diagnostic or therapeutic procedures.
Standard Curve Range
25-1600 pg/mL
Sensitivity
10 pg/mL
Reproducibility
Intra-assay CV: ≤10%; Inter-assay CV: ≤15%
Contents of kit
Reagents Quantity Reconstitution
Cytokine standard 2 vials Lyophilized, reconstitute with the volume of reagent indicated on the vial
Biotinylated antibody 2 vials Dilute 100 times in Diluent buffer(1×)
Streptavidin-HRP 2 vials Dilute 100 times in Diluent buffer(1×)
Diluent buffer(1×) 3 vials Ready-to-use
Washing buffer(50×) 1 vial Dilute 50 times in distilled Water
TMB 1 vial Ready-to-use
Stop solution 1 vial Ready-to-use
Precoated ELISA plates 8×12 Ready-to-use
Plastic cover 4 sheets Ready-to-use
Plate
Pre-coated ELISA Strip plate
Storage
Store the unopened kit at 2-8℃, The kit is stable for 12 months. Do not use beyond expiration. Keep sterile once opened.