The Rat TGF-β1 ELISA kit is to be used for the in-vitro quantitative determination of TGF-β1 in rat serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant rat TGF-β1 .
This kit has been configured for research use only and is not to be used in diagnostic or therapeutic procedures.
Sample
Rat serum, plasma, buffered solutions or cell culture medium
Standard Curve Range
15.6-1000 pg/mL
Sensitivity
5pg/mL
Reproducibility
Intra-assay CV: ≤10%; Inter-assay CV: ≤15%
Contents of kit
Reagents Quantity Reconstitution
Cytokine standard 2 vials Lyophilized, reconstitute with the volume of reagent indicated on the vial
Biotinylated antibody 2 vials Dilute 100 times in Diluent buffer(1×)
Streptavidin-HRP 2 vials Dilute 100 times in Diluent buffer(1×)
Diluent buffer(1×) 3 vials Ready-to-use
Washing buffer(50×) 1 vial Dilute 50 times in distilled Water
TMB 1 vial Ready-to-use
Stop solution 1 vial Ready-to-use
Precoated ELISA plates 8×12 Ready-to-use
Plastic cover 4 sheets Ready-to-use