he Rat IL-6 ELISA kit is to be used for the in-vitro quantitative determination of IL-6 in rat serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant rat IL-6 .
This kit has been configured for research use only and is not to be used in diagnostic or therapeutic procedures.
Standard Curve Range
31.3-2000 pg/mL
Sensitivity
10 pg/mL
Reproducibility
Intra-assay CV: ≤10%; Inter-assay CV: ≤15%
Plate
Pre-coated ELISA Strip plate
Storage
Store the unopened kit at 2-8℃, The kit is stable for 12 months. Do not use beyond expiration. Keep sterile once opened.
Contents of kit
Reagents - Quantity - Reconstitution
Cytokine standard - 2 vials - Lyophilized, reconstitute with the volume of reagent indicated on the vial
Biotinylated antibody - 2 vials - Dilute 100 times in Diluent buffer(1×)
Streptavidin-HRP - 2 vials - Dilute 100 times in Diluent buffer(1×)
Diluent buffer(1×) - 3 vials - Ready-to-use
Washing buffer(50×) - 1 vial - Dilute 50 times in distilled Water
TMB - 1 vial - Ready-to-use
Stop solution - 1 vial - Ready-to-use
Precoated ELISA plates - 8×12 - Ready-to-use
Plastic cover - 4 sheets - Ready-to-use