The kit includes detection antibody, streptavidin-HRP, AEC coloring system, dilution buffer, Cell stimulator ,washing buffer and multifilter plate that is pre-coated with captured antibody. The kit is a ready-to-use and quality assurance product for all needs of ELISPOT assay. The performance of the kit is carefully examined to other comparable ELISPOT kits world wide to assure equivalent sensitivity, stability, and reproducibility.
Contents of kit
Item - Quantity
Biotinylated antibody - 100μL
Streptavidin-HRP - 100μL
Cell stimulator - 50T
Dilution buffer R(1×) - 10mL×2
Washing buffer (50×) - 15mL
AEC dilution - 10mL
AEC solutionⅠ(20×) - 500μL
AEC solutionⅡ(20×) - 500μL
AEC solution Ⅲ(200×) - 50μL
Precoated PVDF plate - 1plate
Substrate
Streptavidin-HRP
Storage
Store the unopened kit at 2-8ºC. The kit is stable for 12 months. Do not use beyond expiration. Keep sterile once opened.