Automatic sample preparation system AP 22 IF BLOT ELITE

ELISA testfor immunofluorescence assaysfor blot transfer

AP 22 IF BLOT ELITE is a full automated system for ELISA and IFA and BLOT methods. The instrument is able to run 2 ELISA micro-plates with 8 tests on line, 16 IFA slides or 24 BLOT strips; besides of ELISA and IFA processing (AP22, AP22 IF), it is able to carry out the full automation for the Dot/Western Blot, thanks to the blot module and a camera, starting from sample dilution and dispense, reagent dispense, incubation, strip washing to the dot imaging processing and assessment for each strip; the software also provides the data record and patient report. ELISA technical features ELISA dilution cups - 110 positions Removable trays - Modulare rack to hold calibrators, controls and reagents for 8 tests on line Microtiter plates - 2 standard plates (12 x 8 well format) incubated at room temperature or from 36° to 45°C Shaker - Microtiter plate shaking capability (optional) Liquid handling - 1 probe for dilution and dispense Sample dispense time - 11s with 2ml of wash buffer Plate washing - Two 8 nozzles manifold, one to dispense and the second to aspirate; residual volume <3µl Wash buffers - 2 Buffers for Elisa microplates in external tanks with level sensors Plate washing inaccuracy - CV <5% with 300 µl Reading system - 8 independent photo-metric channels with mono and bichromatic reading capability Interferential filters - 2 precision filters (450 e 630 nm); 2 more on request (4 filters max; 400-700 nm) Reading capability - Reading from 0.000 a 3.000 OD; inaccuracy ± 1% from 0.000 to 1.500 OD ± 2% from 1.500 to 3.000 OD IFA technical features Liquid handling - 2 probes: one to dilute and dispense, the second for slide washing