DeNovix CellDrop Assay Kits are simple to use and designed for Brightfield and Fluorescence applications preloaded on CellDrop instruments. DeNovix offers multiple assay kits for cell counting viability assessment, including Acridine Orange / Propidium Iodide (AO/PI) for the CellDrop FL Fluorescence Cell Counter.
Optimized for CellDrop Applications
Cell Counting and Viability
Rapid Incubation
Integrated into EasyApps™ Software
Simple Mix-and-Measure Assay
AO-PI Viability Assay
The Acridine Orange (AO) / Propidium Iodide (PI) assay is used for cell counting and viability. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. All live, nucleated cells fluoresce green due to AO, while dead, nucleated cells are stained with both AO and PI and fluoresce red.
Acridine Orange
The Acridine Orange (AO) Dye is used for total cell counting. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells.
Propidium Iodide
The Propidium Iodide (PI) Dye is used for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells.
Yeast
The Fluorescein Diacetate (FDA) and Propidium Iodide (PI) assay is used for yeast cell viability testing. FDA is a cell viability probe that is membrane-permeable to live yeast cells.